Immobilization of Lipases on Chitosan Hydrogels Improves Their Stability in the Presence of the Products of Triglyceride Oxidation

A significant bottleneck for the industrial application of lipases stems from their poor stability in presence commercial triglycerides. This is mainly due to inactivating effect products triglyceride oxidation (PTO), which are usually produced when oils and fats, being imported far countries, stored long periods. In this study, immobilization a lipase Candida rugosa on chitosan hydrogels has been carried out following two alternative approaches based enzyme adsorption entrapment increase under operating conditions that typical oleochemical transformations. The model compounds representing different classes PTO studied optimize method. Particular attention devoted characterization nonaqueous media, adopted most applications lipases.